Stickiness and clumping of the chromosomes were some of the most common effects
of these tested compounds on the treated root tips. Stickiness usually leads to the formation of anaphase and telophase bridges, and this ends up inhibiting post-telophase, metaphase and cytokinesis, CB-5083 manufacturer respectively, and thus hampering cell division. In vitro cytotoxicity activity by MTT assay method All the synthesized compounds prepared by Scheme I and previously reported (Chhajed et al., 2007, 2013) compounds were subjected to anticancer activity. CTC50 (cytotoxic concentration at which 50 % of the cells are dead after drug exposure) determined for test and standard compound with the help of MTT assay HEK 293 (epidermal kidney cell line), BT474 (breast cancer cell line) and Crenigacestat research buy NCI-H226 (lung cancer) cell lines by MTT method (Freshney, 2000; Edmondson et al., 1988; Prasad et al., 2005; Chiruvella et al., 2008). The viability of control cells was designated as 100 %, and the others were expressed as percentage compared to the control.
The results were compared with standard drug indisulam (ISL). The results demonstrated a strong dose-dependent growth inhibition in treated cell lines. It showed that different cells had a different sensitivity to the inhibition effect of tested compounds. The results are given in Tables 3, 4 and 5 for HEK 293, BT474 and NCI-H226. Thus, from the data, it can be concluded that all test compounds are potent Selleck Mocetinostat cytotoxic agents because of higher CTC50 at lower concentrations, and moreover, the compound (4b) (CTC50 = 0.922) and compound (7f) (CTC50 = 0.754) were found to be most potent agent among all the compounds tested against HEK 293. While compounds
(9c) (CTC50 = 0.751) and (9j) (CTC50 = 0.913) were found to be most potent agent among all the compounds G protein-coupled receptor kinase tested against BT474 and NCI-H226 cell lines, respectively. But none of tested compound was found to be potent compared to standard drug indisulam. From above all cell lines such as HEK 293, M468 and NCI-H226, it has been concluded that compounds (7f), (6f), (9b), (9c) and (9j) are more potent than all synthesized compounds. Compounds (6e) and (6b) have moderate activity than all synthesized compounds. Compounds (4a) and (9 g) have less activity than all synthesized compounds on all cell lines. Structure activity relationship of compounds showed that the presence of NH linker between aryl moiety which is substituted by electron-withdrawing group and 1,3,4-thiadiazole ring has been recognized as potent anticancer agent. Substitution on phenyl ring with chloro, methoxy and nitro group gives better anticancer activity.