baseline seronegative subjects (Table 4), and subjects who were baseline seropositive demonstrated 36 month antibody levels similar to those achieved by baseline seronegative subjects. Baseline HPV 16 DNA positive vs. negative subjects had this website similar

36 month antibody levels, whereas 36 month antibody levels for HPV 18 DNA positive vs. negative subjects were approximately 2- to 3-fold higher. However, this difference did not achieve statistical significance (Table 5). Among subjects enrolled in this 2-dose vs. 3-dose Q-HPV vaccine trial, HPV 16 antibodies measured by the cLIA, TIgG and PsV NAb assays remained detectable for at least 36 months for all subjects. In contrast, beginning at 18 months post-vaccine, the cLIA was unable to detect HPV 18 antibodies in a subset Selumetinib in vitro of subjects, while HPV 18 antibodies remained detectable for at least 36 months in most subjects by the TIgG assay and in all subjects by the PsV NAb assay (NTpartial endpoint). Other studies have demonstrated that up to 40 percent of vaccinated subjects lose detectable

HPV 18 cLIA antibodies over time, but vaccine efficacy in preventing subsequent HPV 18 infection is maintained [4], [5] and [6]. Consistent with our observations, when such individuals are tested by the TIgG [15] or a PsV NAb assay [16], HPV 18 antibodies remain detectable in the majority of individuals for at least 48 months. We demonstrated that HPV 16 and HPV 18 antibody titres reach a plateau about 18 months post-vaccine for both 2- and 3-dose regimens, and remain essentially unchanged through to 36 months. This is encouraging from a public health perspective and suggests that detectable antibodies may be maintained long-term following a 2-dose vaccine schedule because in young girls. Correlation coefficients for HPV 18 for all three assays were very similar, whereas for HPV 16, correlation between the PsV NAb and the TIgG assay was closer than either the PsV NAb or TIgG assays vs. the cLIA. There were a number of

subjects with low levels of HPV 16 cLIA antibodies who displayed high levels of PsV NAb. For HPV 18, the cLIA and PsV NAb were more closely correlated. For those samples which lost detectable HPV 18 cLIA antibodies, the corresponding PsV NAb levels were typically low, confirming the close correlation. These findings likely reflect the more limited array of HPV antibodies detected by the cLIA due to its monoclonal antibody design or may reflect the composition of the PsV. Of interest, Hernandez et al. reported that HPV 16 antibodies detected by enzyme immunoassay (EIA) against either L1 or L1-L2 VLPs correlated well with the results of a PsV NAb assay. However, for HPV 18, EIA antibodies against L1-L2 VLPs correlated better with the PsV NAb assay than EIA antibodies against L1 VLPs. These authors suggest that L1-L2 VLPs likely more closely resemble native virions than L1 VLPs [17].