Conclusion We demonstrated that, SPEF with high Bucladesine ic50 repetition frequency could also achieve similar levels of in vitro and in vivo antitumor efficiency which could be used to reduce unpleasant sensations that occurred in tumor electrical treatment. In addition, rich components of nanosecond pulse contained in SPEF with high frequency electromagnetic fields (5 kHz) could induce cell apoptosis and provided complementary antitumor effect for

the marginal regions with weak electric fields. Our research proposed potential applications and feasibility of using high frequency SPEF in clinical cancer treatment. Nevertheless, it should be noted that this study examined only in vitro and in vivo antitumor effect of SPEF with various frequencies. However, effects of pulse repetition frequencies on biomechanical properties of skeletal selleck products muscle and on pain perception threshold remained to be further clarified. In future study, in order to integrate current antitumor investigation with biomechanical experiment and extend its perspective clinical applications, we should take this limitation into consideration and try to perform in vivo biomechanical test and pain threshold measurement in response to SPEF with different frequencies. Acknowledgements This study was supported by Zhejiang

Provincial Natural Science Foundation of China (to Xiao-Jun Yang) (General Program, Project No. Y206482). Moreover, it was also sponsored in part by two grants from the National Natural Science Foundation of China (Key Program to Cai-Xin Adenosine triphosphate Sun, Project No.50637020 and General Program to www.selleckchem.com/products/cbl0137-cbl-0137.html Li-Na Hu, Project No.30371619). References 1. Weaver JC: Electroporation of biological membranes from multicellular to nano scales. ITDEI 2003, 10: 754–768. 2. Weaver JC: Electroporation: a general phenomenon for manipulating cells and tissues. J Cell Biochem 1993, 51: 426–435.PubMed 3. Gothelf A, Mir LM, Gehl J: Electrochemotherapy: results of cancer treatment using enhanced delivery of bleomycin by electroporation. Cancer Treat Rev 2003,

29: 371–387.CrossRefPubMed 4. Davalos RV, Mir IL, Rubinsky B: Tissue ablation with irreversible electroporation. Ann Biomed Eng 2005, 33: 223–231.CrossRefPubMed 5. Edd JF, Horowitz L, Davalos RV, Mir LM, Rubinsky B: In vivo results of a new focal tissue ablation technique: irreversible electroporation. IEEE Trans Biomed Eng 2006, 53: 1409–1415.CrossRefPubMed 6. Rubinsky B: Irreversible electroporation in medicine. Technol Cancer Res Treat 2007, 6: 255–260.PubMed 7. Schoenbach KH, Hargrave B, Joshi RP, Kolb JF, Nuccitelli R, Osgood C, Pakhomov A, Stacey M, Swanson RJ, White JA, et al.: Bioelectric effects of intense nanosecond pulses. ITDEI 2007, 14: 1088–1109. 8. Mi Y, Sun C, Yao C, Li C, Mo D, Tang L, Liu H: Effects of steep pulsed electric fields (SPEF) on mitochondrial transmembrane potential of human liver cancer cell. Conf Proc IEEE Eng Med Biol Soc 2007, 2007: 5815–5818.PubMed 9.