In all these cases, a hupK-like gene was identified in the DNA re

In all these cases, a hupK-like gene was identified in the DNA region between hupF and hypC (Table  1) suggesting a structure for hydrogenase gene clusters similar to that described for R. leguminosarum[15]. Interestingly, all organisms encoding the three HupF, HypC and HupK proteins were able to express hydrogenase in the presence of oxygen. Anaerobic bacteria (sulphate-reducers and other anaerobes) encoded only one hypC/hupF-like gene, and no hupK-like gene, and the same situation was found

in Enterobacteriaceae. Table 1 Location of genes encoding HupL, HupF, HupK, and HypC proteins in genomes from Proteobacteria Bacterial species #a KEGGbLocus designation for homolog to     HupL HupF HupK HypC Alkalimnicola ehrlichei 1 Mlg_2028

Mlg_2025 Mlg_2020 Mlg_2016 Azoarcus sp. BH72 2 azo3787 azo3793 azo3798 azo3802 Azotobacter vinelandii 3 Avin_50580 Avin_50550 Avin_50500 Avin_50460 Beijerinckia indica 4 Bind_1151 PRI-724 ic50 Bind_1154 Bind_1158 Bind_1162 Bradyrhizobium sp. ORS278 5 BRADO1685 BRADO1688 BRADO1693 BRADO1698 Bradyrhizobium japonicum USDA110 6 bsl6941 bsl6938 bll6933 bsl6929 Bradyrhizobium sp. BTAi1 7 BBta_1997 BBta_2000 BBta_2005 BBta_2009 Burkholderia vietnamiensis 8 Bcep1808_5932 Bcep1808_5935 Bcep1808_5940 Bcep1808_5944 Burkholderia phymatum 9 Bphy_7264 Bphy_7261 Bphy_7257 Bphy_7253 Dechloromonas aromatica Selleck MRT67307 10 Daro_3988 Daro_3985 Daro_3980 Daro_3967 Magnetococcus sp. 11 Mmc1_2503 Mmc1_2501 Mmc1_2497 Mmc1_2490 Magnetospirillum magneticum 12 amb1647 amb1645 amb1644 amb1640 Methylibium petroleiphilum 13 Mpe_A2826 Mpe_A2821 Mpe_A2817 Mpe_A2813 Paracoccus denitrificans 14 Pden_3098 Pden_3102 Pden_3106 Pden_3110 Polaromonas naphtalenivorans 15 Pnap_1974 Pnap_1970 Pnap_1965 Pnap_1961 Ralstonia metallidurans 16 Rmet1297 Rmet1292 Rmet1287 Rmet1283 Rhodobacter sphaeroides ATCC17029 17 Rsph17029_2147 Rsph17029_2151 Rsph17029_2155 Rsph17029_2159 Rhodoferax ferrireducens 18 Rfer_4091 Rfer_4093 Rfer_4118 Rfer_4098 Rhodopseudomonas palustris 19 RPA0963 RPA0967 RPA0972

RPA0976 Rhodospirillum rubrum ATCC11170 20 Rru_A1162 Rru_A1165 Rru_A1167 Rru_A0307 Xanthobacer autotrophicus 21 Xaut_2174 Xaut_2177 Xaut_2181 Xaut_2185 aTaken from SPTBN5 KEGG gene https://www.selleckchem.com/products/Romidepsin-FK228.html database ( http://​www.​genome.​jp/​kegg/​genes.​html). bOrdinal numbers used in phylogenetic tree of Figure  1. The availability of the 3D structure of HypC from Thermococcus kodakarensis[25] allowed us to model both R. leguminosarum HypC and HupF proteins on that template (Figure  1A). We found that the model derived for HupF is compatible with a structure highly similar to that of HypC, except for the C-terminal domain present only in HupF (Figure  1C). This structural similarity suggests a related function for both proteins. Figure 1 Structural, phylogenetic, and sequence comparisons of HupF and HypC. A) Overlay of HupF (white) and HypC (blue) predicted structures.

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