Statistical Analysis By using the power static software collection (SSC), with a power of 80%, an α level of 0.05, consideration of variance 15 and mean difference 10 in BE, the appropriate sample size for each group was determined

to be at least 34 patients (total of 68 patients). Analysis was performed using SPSS 14.0 version for Mac OS X. Discrete variables were compared using the chi-square Inhibitors,research,lifescience,medical and Fisher’s exact tests using a permutation method for multiple testing. Continuous variables were compared by the independent t-test and repeated measures analysis of variance. All values have been presented as mean±SD and P<0.05 was considered significant. Results From 94 patients with end-stage liver disease who underwent orthotropic deceased donor liver transplantation from February 2010 to September 2010, 75 were eligible for this study. There were 37 patients assigned to the restricted normal saline group and 38 in the non-restricted normal saline group Inhibitors,research,lifescience,medical (figure 1). Figure 1 Flowchart of patients according to the consort guideline There were no significant differences in the demographic characteristics of the donors and the recipients (P>0.05; tables 1 and ​and2)2) or in the graft factors between both groups (P>0.05; table 2). We observed no significant

differences in the baseline mean arterial Inhibitors,research,lifescience,medical pressure (P=0.22), the baseline heart rate (P=0.71) and the baseline CVP (P=0.32) between the two groups (figure 2). Table 1 Demographics characteristics of participants Table 2 Demographic characteristics of donor groups Figure 2 Mean changes in Inhibitors,research,lifescience,medical heart rate, central venous pressure (CVP) and mean arterial pressure from baseline to reperfusion in the non-restricted normal saline (NRF) group and restricted normal saline (RF) group. T1: After skin incision; T2: 15 min before reperfusion; Inhibitors,research,lifescience,medical … There were no significant differences in estimated blood loss and use of blood products during surgery between the two groups (P>0.05; table 3).

Table 3 Blood and blood component therapy in both groups During the hepatectomy, anhepatic and neo-hepatic phases, Bumetanide the restricted normal saline group had lower CVP compared to the non-restricted normal saline group (P=0.002; figure 2). However, no significant differences were found between the two groups for urine output (ml/kg/h) during the three phases of OLT (P>0.05; table 4). In addition, during these phases there were no significant differences in mean arterial pressures and the heart rates between the two groups (P=0.34, P=0.47 respectively; figure 2). Table 4 Urine output during the three PXD101 order stages of liver transplantation We observed no significant differences in baseline arterial blood pH (P=0.32), HCO3 (P=0.47), and BE (P=0.67; figure 3).