Table 4 Nucleotide content and gene count levels of the genome Figure 6 Graphical circular such map of the chromosome. From the outside in, the outer two circles show open reading frames oriented in the forward (colored by COG categories) and reverse (colored by COG categories) directions, respectively. The third circle marks … Table 5 Number of genes associated with the 25 general COG functional categories Comparison with the genomes from other Peptoniphilus species Here, we compared the genome sequence of P. obesi strain ph1T with those of P. harei strain ACS-146-V-Sch2b, P. lacrimalis strain 315-B, Peptoniphilus senegalensis JC140T, Peptoniphilus timonensis JC401T, Peptoniphilus grossensis ph5 T and Peptoniphilus indolicus strain ATCC BAA-1640. The draft genome sequence of P.

obesi strain ph1T has a larger size than that of P. lacrimalis (1.69Mb) and P.timonensis (1.76Mb), but a smaller size than that of P. harei (1.83Mb), P. grossensis (2.10Mb), P. senegalensis (1.84Mb) and P. indolicus (2.20Mb). The G+C content of P. obesi is comparable to that of P. lacrimalis and P. timonensis (30.10%, 29.91% and 30.70% respectively) but less than that of P. harei (34.44%), P. grossensis (33.90%), P. senegalensis (32.20%) and P. indolicus (32.29%) P. obesi has more predicted ORFs than P. lacrimalis, (1,718 vs 1,586) but fewer than P. harei, P. senegalensis, P. timonensis, P. grossensis and P. indolicus (1,725, 1744, 1922, 2041 and 2262, respectively). In addition, P. obesi shared 931, 957, 967, 1019, 1055, 1077 orthologous genes with P. indolicus, P. timonensis, P. lacrimalis, P.

senegalensis, P. harei and P. grossensis, respectively. The average nucleotide sequence identity ranged from 69,14% to 87,28% among Peptoniphilus species, and from 71,04 to 71.80% between P. obesi and other species, thus confirming its new species status. Table 6 summarizes the numbers of orthologous genes and the average percentage of nucleotide sequence identity between the different genomes studied. Table 6 Number of orthologous genes (upper right) and average nucleotide identity levels (lower left) between pairs of genomes determined using the Proteinortho software [41]. Conclusion On the basis of phenotypic (Table 2), phylogenetic and genomic analyses (Table 6), we formally propose the creation of Peptoniphilus obesi sp. nov. that contains the strain ph1T.

This strain Brefeldin_A has been found in Marseille, France. Description of Peptoniphilus obesi sp. nov. Peptoniphilus obesi (o.be.si. L. masc. gen. adj. obesi of an obese, the disease presented by the patient from whom the type strain ph1T was isolated). Colonies are 0.4 mm in diameter on blood-enriched Columbia agar and stain gray, transparent, opaque, colonies are not bright. Cells are coccoid, diameter range from 0.77��m to 0.93 ��m with a mean diameter of 0.87 ��m.Optimal growth is achieved anaerobically. No growth is observed in aerobic conditions.