The capsular polysaccharide shows among the most important pneumococcal virulence facets and is differentially regulated in various number habitats. Different phenotypes of the virus bring about colonization, survival, or distribution. Several studies have suggested that the pill prevents addition of pneumococci to epithelial cells, as well as to endothelial cells. Although the opaque phenotype is more controversial in systemic infections, the clear phenotype, natural product library which produces smaller levels of capsular polysaccharide, was shown to be more successful in colonizing mucosal surfaces of the nasopharynx and in residing on surfaces. In epidemiological studies nontypeable, nonencapsulated nasopharyngeal carrier strains were identified, and one number of these organisms was genetically closely associated with encapsulated strains. As well as elucidation of gene expression profiles during pathogenesis, it’s necessary to visualize phenotypic changes of subcellular components during infectious processes. Urogenital pelvic malignancy The examination of phenotypes should provide insights to the mechanism facilitating adaptation of pathogens for their host niches. In this study the capsular polysaccharides of various pneumococcal serotypes were evaluated in vitro and in vivo using a modified fixation method for electron microscopic studies which preserved the capsular material. Differences in the amount of capsular polysaccharide were shown to influence adherence and invasion significantly. The capsular polysaccharide is highly hydrated and includes numerous anionic charged websites. Ruthenium red is used previously to visualize the capsule of S. pneumoniae and Klebsiella pneumoniae. Nonetheless, the fixation process stated earlier led to inadequate stabilization of the pneumococcal capsule. A lysine based aldehyde met inhibitor ruthenium red fixation project led to very stable maintenance of the staphylococcal glycocalyx. This LRR fixation protocol resulted in greatly increased preservation of the pneumococcal capsule and decreased the fibrous and somewhat fuzzy appearance of the capsule observed in the lack of lysine. Because of this, the LRR fixation method allowed for initially observation of the active process of capsule expression around the bacterial surface of fixing and entering pneumococci by high resolution FESEM, thus discriminating between extremely encapsulated and weakly encapsulated bacteria. There is no dependence on pill specific antibodies, and the strategy may be applied to all pneumococcal serotypes. More over, this fixation technique may also be employed to preserve and stabilize polysaccharides of other infections, including Streptococcus pyogenes. When the LRR fixation method is used, the width of bacterium related carbohydrate structures could be monitored.