The committee acknowledges the need for further study of protein requirements for extreme, military-specific environmental conditions and whether unique metabolic stressors associated with military service alter protein requirements for aging warfighters.”
“Members of the pathogenic Legionella genus

encounter suitable growth conditions in nuclear power plant cooling circuits. To limit its proliferation and ensure that levels remain below regulatory thresholds, chemical treatment with monochloramine can be used in continuous or sequential conditions. The aim of this study was to determine the impact of PX-478 monochloramine on L.pneumophila subpopulations in the cooling circuits of a nuclear power plant. The chosen procedure involved monitoring the diversity and dynamics of L.pneumophila subpopulations every month over the course of a year in a nuclear power plant cooling circuit, which was treated for 2months during the period under study. This study confirmed the effectiveness of monochloramine to limit L.pneumophila concentrations in cooling circuits. The culturable L.pneumophila community was strongly affected by the injection of monochloramine. Several subpopulations persisted during treatment at low concentrations (below the detection limit of

standard methods), buy MRT67307 suggesting that the susceptibility of L.pneumophila is strain dependent. Although the composition of the subpopulations was not similar, the resilience of the community structure was observed. Indeed, the community eventually returned to its initial structure p38 MAPK phosphorylation and presented a similar pattern of richness, diversity

and uniformity to that seen before treatment.”
“We characterized the Schizosaccharomyces pombe arc3 gene, whose product shares sequence homology with that of the budding yeast ARC18 and human ARPC3/p21 subunits of the Arp2/3 complex. Our data showed that Arc3p co-localizes with Factin patches at the cell ends, but not with F-actin cables or the equatorial actin ring, and binds other subunits of the Arp2/3 complex. Gene deletion analysis showed that arc3 is essential for viability. When arc3 expression was repressed, F-actin patches became dispersed throughout the cell with greatly reduced mobility. Furthermore, in arc3-repressed cells, endocytosis was also inhibited. Human ARPC3 rescued the viability of the Sz. pombe arc3 null mutant; in addition, ARPC3 also localized to F-actin patches in human cells. These data suggest that Arc3p is an evolutionarily conserved subunit of the Arp2/3 complex required for proper F-actin organization and efficient endocytosis. Copyright (C) 2011 John Wiley & Sons, Ltd.”
“Non-photochemical quenching (NPQ) of chlorophyll a fluorescence and leaf gas-exchange were investigated in relation to the chlorophyll and carotenoid content, and the xanthophyll cycles in wild type tomato (Solanum lycopersicum, L cv Red Setter (RS)) and in two transgenic lines (UO and UU) over-expressing beta-carotene hydroxylase.