The expression of this construction in strain E coli BL21(DE3) w

The expression of this construction in strain E. coli BL21(DE3) was studied and conditions of synthesis, isolation, and purification of recombinant protein were optimized. The hydrodynamic radius of hybrid protein was determined by the method of dynamic diffusion. Energy transfer between sensitized terbium and red fluorescent protein was confirmed by the methods of fluorescence spectroscopy. The obtained FRET-pair may be used both for studies in vitro and as reporters

in living cells.”
“OBJECTIVE: Although hydrocodone-acetaminophen is commonly used for pain control in first-trimester abortion, the efficacy of oral opioids for decreasing pain has not been established. Our objective was to estimate the effect of hydrocodone-acetaminophen on patient pain perception during first-trimester surgical abortion.

METHODS: We conducted a randomized, double-blinded, placebo-controlled trial. Patients (before 11 weeks of HSP990 concentration gestation) received standard premedication (ibuprofen and lorazepam) and a paracervical block with the addition of 10 mg hydrocodone and 650 mg acetaminophen or placebo 45-90 minutes before surgical abortion. A sample size of 120 was calculated

to provide 80% power to show a 15-mm difference (alpha=0.05) in the primary outcome of pain with uterine aspiration (100mm visual analog scale). Secondary outcomes AC220 supplier were pain at additional time points, satisfaction, side effects, adverse events, and need for additional pain medications.

RESULTS: There were no significant differences in demographics or baseline pain between groups. There were no differences

in pain scores between patients receiving hydrocodone-acetaminophen compared with placebo during uterine aspiration (65.7 mm compared with 63.2 mm, P=.59) or other procedural time points. There were no differences KU-57788 in vitro in satisfaction or need for additional pain medications. Patients who received hydrocodone-acetaminophen had more postoperative nausea than those receiving placebo (P=.03) when controlling for baseline nausea. No medication-related adverse events were noted.

CONCLUSION: Hydrocodone-acetaminophen does not decrease pain during first-trimester abortion and may increase postoperative nausea.”
“The connection between the efficiency of phenolic lipids and their hydrophobic property (solubility) and hydrophobic property of microorganisms’ cell structure is shown. The mixture of amphiphilic di(oxiphenil)-phenil-methanes, which act bacteriostatically under 15 mg/l, possesses maximal efficiency against Staphylococcus aureus. Against Mycobacterium smegmatis with hydrophobic cell wall, hydrophobic 2,4-dialkylocibenzol 70 mg/l was the most effective. Hexylresorcin (HR) stops the development of gram-positive bacteria in concentrations 20-50 mg/l, that of gram-negative bacteria in concentration 65 mg/l, that of M. smegmatis at 70 mg/l, and that of yeast and fungi at 300 mg/l. HR prevails bacteria spores germination in the concentration 25-100 mg/l.

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