The growth curve of primary mTOR inhibitor breast transplanted tumors showed that the average tumor volume of the mice in the control SRT1720 and UTI groups was not markedly reduced; however, UTI delays the increase in transplanted tumor volume (P < 0.05). In contrast, the average tumor volume in animals in the TXT and UTI+TXT groups gradually reduced over time after 11 d in the order of UTI+TXT > TXT (P < 0.05). King's formula was q = 1.088, implying an additive inhibitory effect of UTI and TXT

on the growth of transplanted breast cancer in nude mice (Figure 8a). The growth curve of the MDA-MB-231 transplanted tumors was the same (Figure 8b). Figure 8 (a). Growth curve for primary breast cancer cell xenografted tumors. (b). Growth curve for MDA-MB-231 breast cancer cell xenografted tumors. 3.7 Effects of UTI and TXT protein expression of PAFR, PDGFA, IGF-1R, NGF, NF-κB, and JNk-2 in xenografted tumors Immunohistochemistry showed that UTI, TXT, and UTI+TXT significantly inhibited the protein expression of PDGFA, NGF, and IGF-1R compared with the control group (P < 0.05). The inhibitory effect of UTI+TXT was strongest. The expression of ki-67, JNk-2, and NF-κB was reduced in the UTI, TXT, and UTI+TXT

groups; however, the protein expression Ion Channel Ligand Library of caspase-3 increased significantly, and this effect was strongest for UTI+TXT (P < 0.05;Figure 9, 10, 11). Figure 9 Effect of UTI and TXT on the immunohistochemistry expression of IGF-1R, PDGFA, NGF, NF-κB, ki-67, caspase-3, JNk-2 in xenografted tumor tissue of nude mice. Figure 10 Effect of UTI and TXT on the protein expression of IGF-1R, PDGFA, NGF, NF-κB, ki-67, caspase-3, JNk-2 in xenografted tumor tissue of

nude mice. Figure 11 Effect of UTI and TXT on the protein expression of NGF in MDA-MB-231 xenografted tumor tissue of nude mice. 4. Discussion Primary culture is the first culture after obtaining tissue from donor. The advantage of primary culture is that most of the Fossariinae cell still displays the biological characteristics of the in vivo cells. The result from Koechli [8] reported that an in vitro experimental result has good correlation with in vivo chemotherapeutical reactions (sensitivity = 90%, specificity = 86%). Hence, the primary culture method is suitable for investigating differences in the biological features of tumor cells. Proliferation inhibition and apoptosis are key factors in tumor treatment. In the present experiment, the proliferation of primary (ER+) and MDA-MB-231 (ER-) breast carcinoma cells are inhibited in a time-dependent manner. In addition, apoptosis of breast carcinoma cells increase. The anti-tumor effect of UTI+TXT was stronger than when UTI or TXT was used alone. Thus, UTI can enhance the anti-tumor effect of TXT. ki-67 antigen is a nuclear antigen related to cell proliferation; its function is related to chromosomes and cell karyokinesis [9].