the misuse and over-use of antibacterial agents have triggered the alarming increase of antibiotic resistant strains. Of the important minerals for the cycles of both saturated and unsaturated fatty acids biosyntheses in FAS II, hydroxyacyl ACP has attracted close attention as a vital target for the development of effective anti bacterial ingredients against pathogenic bacteria. Recently, FabZ from H. pylori order Fostamatinib strain SS1 was purified and cloned. The crystal structures of HpFabZ and the further HpFabZ enzymatic characterization and its processes with two inhibitors have provided valuable information for HpFabZ focused anti H. pylori adviser development. The natural product Emodin is originally isolated from the rhi zomes of Rheum palmatum. It exists within the roots and bark of several different conventional Chinese medicine supplements and Chinese medi-cal herbs including Rheum officinale Baill, Rhamnus, and Senna. Emodin shows a broad selection of medicinal properties including anticancer, anti-inflammatory, Infectious causes of cancer antiproliferation, and vasorelaxant actions. It has been noted that Emodin has a regulatory impact on the proliferation of human primary T lymphocyte and immune responses in human mesangial cells, inhibits the proliferation of pancreatic cancer mobile through apoptosis induction related mechanism, accelerates osteoblast differentiation through phosphatidylinositol 3 kinase activation and bone morphogenetic protein 2 gene expression. It may also inhibit the growth of neuroectodermal cancer and breast cancer by suppressing HER 2/neu tyrosine kinase activity in HER 2/neu overexpressing Ubiquitin conjugation inhibitor human breast and lung cancer cells, inhibit tyrosine kinase mediated phosphorylation of vascular endothelial growth factor receptors in colon cancer cells, increase the restoration of nucleiotide removal to the DNA damage of human cells induced by UV and cislatin induction, and eventually competitively block the activity of casein kinase II. In addition, Emodin was previously reported to show inhibitory activity from the expansion of Helicobacter pylori by causing dosedependent DNA damage. However, no acting target information for Emodin inhibition against H. pylori is revealed up to now. In our work, we noted that Emodin functioned like a competitive inhibitor against HpFabZ. So that you can further study the inhibitory mechanism, the thermodynamic and kinetic characterization of Emodin/HpFabZ interaction was examined by surface plasmon resonance and isothermal titration calorimetry based assays. In addition, the crystal structure of HpFabZEmodin comple was also decided to inspect Emodin/ HpFabZ holding at atomic level.