The serotonin syndrome includes a constellation of full body motor responses, but because we were considering reorganization after spinal injury, we focused on motor behavior stated caudal to the injury, i. e., hindlimb activation, which contains coordinated hindlimb significant with alternating rhythmic activities. Animals were seen in the home cage after drug administration and rated on a 4 point scale for every element of the problem. These data are reported while the difference between standard score and drug effect. Data for BBB tests were examined by two way ANOVA between class and drug at both 4 and 12 months post-surgery. Data for BBB checks at week 6 were analyzed by two-way ANOVA between drug and class with drug taken as a repeated measure. Post hoc analysis was performed, where appropriate, using Dunnetts test. Investigation of tremor appearance with time selective FAAH inhibitor was done using the Chi square test. Differences in serotonin syndrome intensities were compared using the paired sign test. All comparisons were regarded as important at the 0. 05 alpha level. Immunocytochemical data from receptor reports were analyzed by ANOVA for the 3 sets of animals with each tissue section taken as someone data point from 3 to 5 replicate slides per animal. Power analyses confirmed a effective reliable variation in these outcome measures Immune system in the 0. 05 alpha level could be determined from 5 to 6 pieces on a minimum of 3 replicate slides each obtained from three animals per group. Benefits Anatomical studies were done caudal to the lesion at both L5 and L2, where CPG nerves and hindlimb motoneurons can be found, respectively. Only L5 data are described below, because data were very similar at both levels. Not surprisingly, longitudinal sections display marked depletion of serotonin caudal to the damage, with some increased 5 HT immunoreactivity just rostral to the lesion. The depletion in 5 HT is higher in the dorsal horn and dorsal lateral funiculus than in the ventral horn and ventral funiculus since the contusion damage is inflicted on the dorsal surface of the back. Quantification of myelin stains has shown that about 3% of axons stay in caudal back following serious contusion and about hundreds of axons survive following a mild contusion. 5 HT axons are among those partly spared by contusion injury, with increased spared by a moderate than the usual serious lesion. We performed a similar quantification of location fraction that price Decitabine was on a the 5 HT immunoreactive axons in the ventral and dorsal horns and the lateral and ventral funiculi. Our results show a of 55% of 5 HT immunoreactivity in the ventral horn of MOD rats and a reduction in serotonergic immunoreactivity in the ventral horn in the lumbar spinal cord of SEV rats.