The strains can be identified by performing tests for LDC and ODC, citrate utilization and acid production from amygdalin, arabinose and sucrose (API 20E system). Based Target Selective Inhibitor Library on these results, strains DY05T and 47666-1 clearly represent a novel species of the genus Vibrio, for which the name V. owensii sp. nov. is proposed. Vibrio owensii (o.wens’i.i. N.L. gen. n. owensii, of Owens, named to honor L. Owens, an Australian microbiologist and specialist in the biology of V. harveyi-related species). Cells are slightly curved Gram-negative rods, 1.0 μm wide × 3.1 μm long, facultative anaerobic

and motile by means of at least one flagellum. After growth for 48 h at 28 °C, the strains form translucent (DY05T) or opaque (47666-1), nonluminescent, nonswarming, smooth and round colonies (2–3 mm) on MA, and bright, yellow and round colonies (2–3 mm) on TCBS agar. Growth occurs in the presence of 1–8% NaCl (w/v), but not at 0% or 10% NaCl. The minimum temperature for growth is 12–15 °C, while the maximum temperature selleck compound for growth is 35–37 °C. No growth occurs at 4 °C. Both strains are ADH-negative, LDC- and ODC-positive. Tests for citrate utilization, production of H2S, urease, Voges–Proskauer, assimilation of arabinose,

and acid production from inositol, sorbitol, rhamnose, melobiose pheromone and arabinose are negative, while tests for nitrate reduction, indole production, tryptophan

deaminase, gelatinase, oxidase, hydrolysis of esculin, assimilation of glucose, mannose, mannitol, potassium gluconate and malate and fermentation of glucose, mannitol, sucrose and amygdalin are positive. Enzyme activities detected by API ZYM tests are alkaline phosphatase, esterase (C4), esterase lipase (C8), leucine arylamidase, α-chymotrypsin, acid phosphatase and naphtol-AS-β1-phosphohydrolase. A difference between strains was seen for the ONPG test, which was positive for 47666-1 and negative for DY05T. Both strains were susceptible to chloramphenicol (30 μg), gentamicin (10 μg), sulphisoxazole (300 μg), trimethoprim-sulphamethoxazole (1/19) (1.25–23.75 μg) and tetracycline (30 μg) and vibriostatic agent O/129 (10 and 150 μg); intermediate to erythromycin (15 μg) and kanamycin (30 μg), and resistant to ampicillin (10 μg). The major fatty acids (>1% for at least one strain) are summed feature 3 (C16:1ω7c and/or C15 iso 2-OH), C16:0, C18:1ω7c, C14:0, C16:0 iso, C12:0, summed feature 2 (C14:0 3-OH and/or C16:1 iso I), C17:0 iso, C17:1ω8c, C17:0, C12:0 3-OH and C18:0. The DNA G+C content is 45.3–45.9 mol%. The type strain is DY05T (=JCM 16517T=ACM 5300T), isolated from cultured larvae of the ornate spiny lobster P. ornatus in Queensland, Australia.