The UV–visible spectrum analysis showed a sharp adsorption peak at ∼439 nm, characteristic of SNPs ( Fig. 1). The typical XRD pattern (Fig. 2) showed diffraction peaks at 2θ = 38°, 44.3°, 64.3°, 77.4° indexed to (111), (200), (220) and (311) planes of silver (JCPDS file no.04-0783) that confirmed the main composition of the nanoparticles was silver. It is evident that SNPs were crystalline

in nature with face Tyrosine Kinase Inhibitor Library centric cubic (fcc) symmetry. The average particle size has been estimated using the Scherrer’s formula: D=0.9λ(βcosθ)where, D is mean crystalline size, β is the full width at half maximum intensity of the peak in radians, λ the wavelength of X-rays (0.1541 nm) and θ is the center angle of the peak in radian. The mean crystalline size for SNPs was determined to be ∼35.42 nm by formula. The SEM images of the nanoparticles synthesized using the culture supernatant were in the size INCB024360 mw range of 30–50 nm (Fig. 3) with uniform arrangement, well dispersed

and spherical in shape. Fig. 4 shows the EDX spectrum where strong signal from Ag was observed and assigned. Peaks for C, O and N correspond to the protein capping over SNPs as evident from FT-IR study (data not shown). In our study, the SNPs exerted a fairly significant antibacterial action on both Gram-negative and Gram-positive bacteria. This is evident from the size of zones of inhibition observed at all concentrations (Table 1) whereas no zone of inhibition was found in the control discs (Fig. 5). This clearly states that the toxicity was induced only by the SNPs producing an average size ranging from 9 to 11 mm Parvulin in a dose dependent manner. The increase in the concentration of SNPs increased the inhibition ability by 1–2 mm. Besides, negative bacteria were found it less sensitive to SNPs than positive bacteria. The genomic DNAs incubated with the SNPs for 6 h and 12 h respectively were analysed for DNA damage (Fig. 6). The control wells showed clear distinct bands in all the four lanes from 2 to 5 run along with a 1 kb DNA marker. Electrophoresis

was performed after 6 h of incubation with SNPs and the band pattern observed. The start of DNA damage could well be appreciated from lane 7 where the band (DNA) was found condensed and localized. It can also be seen in other lanes viz. 6, 8 and 9 likely a smearing pattern resulting in fragmentation showing partial DNA damage. This DNA damage was caused by 1.7 μg/10 μL of SNPs. The results of 12 h incubated DNA with SNPs were compared with the control and 6 h run gel. There is a complete fragmentation of DNA strands as seen in Fig. 7 where only the trail could be observed confirming total DNA damage. The present study focuses on extracellular synthesis of SNPs using a soil isolate B. subtilis A1 and its bactericidal and geno-toxic effects were investigated.