These results exhibit that the captured T cells were well bound on the surface with different morphologies of filopodia or lamellipodia as shown in Figure 2a,b. Interestingly, these images indicate that the morphology (e.g., width of these surface components) BYL719 cost of the captured T

cells is highly correlated with the size of QNPA in diameter from 200 to 450 nm. To ensure the evaluation of the filopodial width in the early stage of cell adhesion, we quantified at least approximately 20 cells. As a result, the widths of filopodia protruding from T cells bound on QNPA were determined to be approximately 69.00 ± 15.10, 71.60 ± 17.1, 104.40 ± 32.50, and 212.50 ± 16.00 nm corresponding to QNPA surface diameters of approximately 100, 200, 300, and 450 nm, respectively, as shown in Figures 2 and 3a. Filopodial morphologies on STR-QNPA below approximately 300 nm in diameter present a long extended shape, but it extends to be remarkably narrow as it has to be confined by adjacent STR-QNPs with 450 nm diameter. We noticed that captured CD4 T cells on the STR-QNPA surfaces exhibited striking differences in morphology on the varied diameters, Luminespib manufacturer even under the condition of extremely early stages of adhesion and statically stable activity of T cells (approximately 20-min Selleckchem Acadesine incubation at 4°C). Furthermore, to assess the significance of our correlation results,

p values were calculated with neighboring column data. Figure 3a exhibits that the distribution of extended filopodial width of the captured CD4 T cells were observed to increase in width by increasing the diameter of QNPA from 200 to 450 nm (**** p < 0.0001, Galeterone Figure 3b,c), resulting

in a good linear response between the width of T cells and diameter of QNPA (R 2 = 0.994, n = 20). On the other hand, the filopodial width for 100-nm QNPA shows a similar trend in size to that of the 200-nm QNPA, exhibiting a statistically insignificant difference (* p = 0.0448, bottom part in Figure 3a,b). Figure 2 SEM images of captured CD4 T cells on four different sizes of QNPA substrates. (a) Top and (b) tilt views. All captured cells were highlighted in blue for easy distinction. Figure 3 Filopodial width distribution, p values, and diagram of CD4 cells bound on four QNPA substrates. (a) Filopodial width distribution of CD4 cells bound on the four different STR-functionalized QNPA substrates after only 20 min of incubation at 4°C. Selected filopodia with distribution (top part of figure) in which only approximately 80% of filopodial width taken from the results (bottom part of figure). (b) Summary of p values for filopodial width distribution of captured CD4 T cells on four different QNPA substrates. p values <0.0001 (****) are considered statistically significant. Less significant statistical difference is represented (* p = 0.0448). (c) Schematic diagram of CD4 T cell spreading mechanism just for 20 min of incubation.