These results were corroborated by bioinformatic

These results were corroborated by bioinformatic HSP activation analysis of the polymyxin synthetase gene cluster in M-1, where the adenylation domains specified the amino acid substrates to be activated (Table 2). This is remarkable, since according to literature, these forms of polymyxin are rare and the fact that all three of the polymyxin gene clusters examined to date are from plant-associated this website strains of P. Table 2 Specificity-conferring amino acids and homologies of the adenylation domains in polymyxin synthetases of strains M-1, E681, and PKB1 Module/ strain Active site residues in A-domain Specified aa % aa E681 % aa PKB1 235 236 239 278 299 301 322 330 Module 1   pmxE1/M-1 D V G E

I S S I L-Dab 99 99 pmxE1/E681 D V G E I S S I L-Dab     pmxE1/PKB1 D V W E I S S I L-Dab     Module 2   pmxE2/M-1 D F W N I G M V L-Thr 99 98 pmxE2/E681 D F W N I G M V L-Thr     pmxE2/PKB1 D F W N I G M V L-Thr     Module 3 (W)   pmxE3/M-1 D V G E I S S I D-Dab 98 92 pmxE3/E681 D V G E I S S I D-Dab     pmxE3/PKB1 D V G E I S S I D-Dab     Module 4   pmxE4/M-1 D V G Flavopiridol (Alvocidib) E I S A I L-Dab 96 96 pmxE4/E681 D V G E I S A I L-Dab     pmxE4/PKB1 D V G E I S A I L-Dab     Module 5   pmxE1/M-1 D V G E I S A I L-Dab

97 89 pmxE1/E681 D V G E I S A I L-Dab     pmxE1/PKB1 D V G E I S A I L-Dab     Module 6 (X)   pmxA1/M-1 D A W T I A A I D-Phe 88 99 pmxA1/E681 D A W I V G A I D-Leu     pmxA1/PKB1 D A W T I A A I D-Phe     Module 7 (Y)   pmxA2/M-1 D F W N I G M V L-Thr 99 51 pmxA2/E681 D F W N I G M V L-Thr     pmxA2/PKB1 D G F L L G L V L-Leu     Module 8   pmxA3/M-1 D V G E I S A I L-Dab 97 92 pmxA3/E681 D V G E I S A I L-Dab     pmxA3/PKB1 D V G E I S A I L-Dab     Module 9   pmxA4/M-1 D V G E I S A I L-Dab 96 91 pmxA4/E681 D V G E I S A I L-Dab     pmxA4/PKB1 D V G E I S A I L-Dab     Module 10 (Z)   pmxB1/M-1 D F W N I G M V L-Thr 97 99 pmxB1/E681 D F W N I G M V L-Thr     pmxB1/PKB1 D F W N I G M V L-Thr     Modules 3 and 6 contained extra epimerization domains which might convert Dab3 and Phe6 to the D-configuration.

Comments are closed.