This work was supported by grants from the National Natural Science Foundation of China (No. 30925002, 30970093 and 30800022) and the National Basic Research (973) Program of China (No. 2010CB126504). Electronic supplementary material Additional file 1: Time course of benzoate consumption and metabolite formation by the wild-type strain A1501. The elution profile of the compounds separated by HPLC is shown. Data in A-C are of samples taken at the indicated times. Conversion of benzoate check details (BEN) to catechol (CAT) and cis, cis-muconate (CCM) by A1501

is indicated by red vertical arrows. (PDF 786 KB) References 1. Harwood CS, Parales RE: The beta-ketoadipate pathway and the biology of self-identity. Annu Rev Microbiol 1996, 50:553–590.PubMedCrossRef 2. Jimenez JI, Minambres B, Garcia JL, Diaz E: Genomic analysis of the aromatic catabolic pathways from Pseudomonas putida KT2440. Environ Microbiol 2002,4(12):824–841.PubMedCrossRef mTOR phosphorylation 3. MacLean AM, MacPherson G, Aneja P, Finan TM: Characterization of the beta-ketoadipate pathway in Sinorhizobium meliloti . Appl Environ Microbiol 2006,72(8):5403–5413.PubMedCrossRef 4. Barbe V,

Vallenet D, Fonknechten N, Kreimeyer A, Oztas S, Labarre L, Cruveiller S, Robert C, Duprat S, Wincker P, MM-102 ic50 Ornston LN, Weissenbach J, Marlière P, Cohen GN, Médigue C: Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium. Nucleic Acids Res 2004,32(19):5766–5779.PubMedCrossRef 5. Butler JE, He Q, Nevin KP, He Z, Zhou J, Lovley DR: Genomic and microarray analysis of aromatics degradation in Geobacter metallireducens and comparison to a Geobacter isolate from a contaminated field site. BMC genomics 2007, 8:180.PubMedCrossRef 6. Salinero Thalidomide KK, Keller K, Feil WS, Feil H, Trong S, Di Bartolo G, Lapidus A: Metabolic analysis of the soil microbe Dechloromonas aromatica str. RCB: indications of a surprisingly complex life-style and cryptic anaerobic

pathways for aromatic degradation. BMC genomics 2009, 10:351.PubMedCrossRef 7. Wu CH, Ornston MK, Ornston LN: Genetic control of enzyme induction in the β-ketoadipate pathway of Pseudomonas putida : two-point crosses with a regulatory mutant strain. J Bacteriol 1972,109(2):796–802.PubMed 8. Houghton JE, Brown TM, Appel AJ, Hughes EJ, Ornston LN: Discontinuities in the evolution of Pseudomonas putida cat genes. J Bacteriol 1995,177(2):401–412.PubMed 9. Cowles CE, Nichols NN, Harwood CS: BenR, a XylS homologue, regulates three different pathways of aromatic acid degradation in Pseudomonas putida . J Bacteriol 2000,182(22):6339–6346.PubMedCrossRef 10. Collier LS, Gaines GL, Neidle EL: Regulation of benzoate degradation in Acinetobacter sp. strain ADP1 by BenM, a LysR-type transcriptional activator. J Bacteriol 1998,180(9):2493–2501.PubMed 11.