tude of change, effects were more pronounced in fish containing lower flesh lipid levels. These results were confirmed by quan tifying the expression of three enzymes catalyzing steps in cholesterol biosynthesis as well as srebp2, a transcription factor that regulates OSI-744 choles terol synthesis. Furthermore, the RT qPCR analysis indicated that this regulation was only associated with lower flesh lipid levels given that in the high lipid group only 7dchr was down regulated. Therefore, this experiment confirmed previous studies suggesting an association between flesh adiposity and n 3 LC PUFA in the regulation of cholesterol biosynthesis in Atlantic salmon families, with lean fish showing a higher re sponsiveness to n 3 LC PUFA.

However, an import ant novel outcome of the present study was the demonstration that the previous results were not solely a consequence of a higher dietary intake of cholesterol supplied by a FO diet in contrast to a VO diet but also resulted from higher incorporation and increased tissue levels of n 3 LC PUFA. The likely explanation for these results is the role of n 3 LC PUFA as regula tors of gene transcription, including some implicated in cholesterol biosynthesis, mediated by srebp2. Nonetheless, the mechanism for why this response was only observed when associated with low flesh lipid levels requires clarification. Recent studies showed that lean humans are also more responsive, in terms of plasma lipid and lipoprotein composition, to cholesterol reducing diets containing lower levels of saturated fatty acids and cholesterol than obese individuals, and several mechanisms have been proposed to explain this.

In the present case, the absolute, rather Entinostat than the relative, level of n 3 LC PUFA may be the determinant factor affecting gene transcription and, in the high lipid group, absolute levels of these fatty acids might have been sufficiently high to repress cholesterol biosynthesis genes, even at lower relative n 3 LC PUFA contents. This hypothesis is supported by the RT qPCR analysis comparing the families with regards to lipid level, HL LL and HH LH. In the HL LL com parison, contrasting absolute n 3 LC PUFA levels of 427 versus 363 mg 100 g flesh, there was down regulation of both ipi and srebp2, whereas comparison of the families HH LH, containing 554 versus 468 mg 100 g flesh, showed no difference in the expression of the genes.

Similarly, genes involved in lipoprotein metabolism, which are also regulated by LC PUFA through different mechan isms, also showed more significant changes when comparing fatter and leaner salmon with lower LC PUFA levels, indicating that a similar regulatory mech Lenalidomide clinical trial anism might occur. Therefore, the present study is consistent with previous work identifying cholesterol and lipoprotein metabolism as pathways significantly and differentially affected by n 3 LC PUFA depending on flesh adiposity. Effects of total lipid level on lipid metabolism Lipid level significantly affected expression of lipid m