Grey and orange bars denote closely located ORFs putatively co-expressed. Homologous coding regions are boxed when a single ORF in one strain corresponds to two or more contiguous ORFs in others. (XLS 998 KB) Additional file 5: Micro-heterogeneity regions. coding regions present/absent in the compared A. baumannnii genomes, denoted in the text as mhrs (micro-heterogeneity regions), and their hypothetical function, are listed in the table. Alternative regions present at the same locus are marked by different colour characters. mhrs Selleckchem EX-527 containing two or more ORFs are boxed. (XLS 123 KB) Additional file 6: Cryptic prophages. structures

of cryptic prophages identified in A. baumannii genomes. Prophage types are boxed to highlight their relatedness as resulting from MAUVE alignment. Different CP1 and CP2 are shown to illustrate the degree of genetic variation of A. baumannii prophage families. (PDF 400 KB) Additional file 7: Gene products putatively encoded by strains 4190, Selleckchem NVP-BGJ398 3909 and 3990. ORFs of strains 4190, 3909 and 3990 and the corresponding contig number are shown. (DOC 4 MB) Additional file 8: Genomic regions, amplified genes, primers, amplicon sizes and cycling conditions used in PCR surveys. (none, title sufficiently describes data). (DOC 90 KB) References 1. Dijkshoorn L, Nemec A, Seifert

H: An increasing threat in hospitals: multidrugresistant Acinetobacter baumannii . Nat Rev Microbiol 2007, 5:939–951.PubMedCrossRef 2. Durante-Mangoni E, Zarrilli R: Global spread of drug-resistant

Acinetobacter baumannii : molecular epidemiology and management of antimicrobial resistance. Future Microbiol 2011, 6:407–422.PubMedCrossRef 3. Nemec A, Krizova L, Maixnerova M, van der Reijden TJ, Deschaght P, Passet V, Vaneechoutte M, Brisse S, Dijkshoorn L: Genotypic and phenotypic characterization Phosphatidylinositol diacylglycerol-lyase of the Acinetobacter calcoaceticus – Acinetobacter baumannii complex with the proposal of Acinetobacter pittii sp. nov. (formerly Acinetobacter genomic species 3) and Acinetobacter nosocomialis sp. nov. (formerly Acinetobacter genomic species 13TU). Res Microbiol 2011, 162:393–404.PubMedCrossRef 4. Higgins PG, Dammhayn C, Hackel M, Seifert H: Global spread of carbapenem-resistant Acinetobacter baumannii . J Antimicrob Chemother 2010, 65:233–238.PubMedCrossRef 5. Van Dessel H, Dijkshoorn L, van der Reijden T, Bakker N, Paauw A, van den Broek P, Verhoef J, Brisse S: Identification of a new geographically widespread multiresistant Acinetobacter baumannii clone from European hospitals. Res Microbiol 2004, 155:105–112.PubMedCrossRef 6. Dijkshoorn L, Aucken H, Gerner-Smidt P, Janssen P, Kaufmann ME, Garaizar J, Ursing J, Pitt TL: Comparison of Smoothened Agonist order outbreak and nonoutbreak Acinetobacter baumannii strains by genotypic and phenotypic methods. J Clin Microbiol 1996, 34:1519–1525.PubMed 7.