Hence the conversion of reducing sugars into ethanol during fermentation was initiated Selleckchem SCH727965 with high inoculums loads of yeast cells. The gross energy value of ethanol produced from the different steam pretreated biomasses at laboratory scale

were 2.21, 1.75, 1.16, 1.69, 1.46 MJ/kg respectively for the A. mangium leaves, A. mangium pods, Ficus leaves, paddy straw and sorghum stubbles. The ethanol-equivalent energy consumption from pretreatment of biomass to ethanol production was equivalent to 0.81 MJ/kg (based on the operating parameters of high-pressure steam vessel and fermentor). The pseudo-net energy value of ethanol produced from the steam pretreated A. mangium leaves, A. mangium pods, Ficus leaves, paddy straw and sorghum stubbles were 1.39, 0.94, 0.34, 0.88, 0.65 MJ/kg of the biomass respectively. The leaves of Acacia showed high net-pseudo energy value and Ficus with less net energy value of ethanol yield. It also suggests though a significant level of energy is consumed for the lignocellulosic ethanol production

from the steam pretreated biomass, it is an indispensable source of alternative buy CHIR-99021 fuel energy. The strong crystalline structure of cellulose, complex hemicelluloses and lignin contents of the crop residues and tree leaf litters limits accessibility of plant biomass to hydrolytic enzymes [32]. Bumetanide However the marine bacterial isolate JS-C42 showed the efficient lignocellulolytic ability to release the reducing sugars from steam pretreated biomass due to the increase in the accessible cellulosic surfaces for the enzymatic actions. Thus the synergistic action of cellulolytic enzymes with the steam pretreated substance

helps in the production of cellulosic ethanol by the substantial release of simple reducing sugars. This study enumerated the release of reducing sugars from the lignocellulosic materials by the subsets of lignocellulolytic enzymes secreted by the bacterial isolate JS-C42 without any external input of commercial enzymes. The average diameter size of the bacterial cells grown on tryptic soy broth without cellulose was 0.117 μM. When the cells grown on Sigmacell cellulose, they were colonized on the surface of the cellulose substrate and they appeared plumpier than the cells grown on tryptic soy broth. The average diameter of cells grown on the microcrystalline cell surface was 0.150 μM. Atomic force microscope image analysis of 12 h grown Isoptericola sp. JSC-42 in the present study revealed the mycelial form ( Fig. 4) with embedded cocci shaped cells appearing like beads on a string arranged in an irregular pattern. The diameter of the cells in the mycelium ranged 0.107–0.264 μm.