The HPLC chromatogram of AG extract is illustrated in Fig. 2. AG include the typical ginsenosides Re, Rg1, Rb1, Rc, Rb2, and Rd (Fig. 2A). After heat processing at 120°C, the ginsenosides

Rb1 and Re were markedly decreased, whereas the peaks of less polar ginsenosides (20(S,R)-Rg3, Rk1, and Rg5) were newly detected at about 23 min and 27 min ( Fig. 2B, Table 1). Therefore, ginsenosides Rb1 and Re were more efficiently deglycosylated and transformed into less polar LGK-974 molecular weight ginsenosides during heat processing than other ginsenosides contained in AG. Fig. 3A shows the morphological changes of human gastric cancer AGS cell treated with AG or HAG. The AGS cell line has been shown to grow in athymic mice and to have the same histochemical and cytological characteristics as the specimen taken from the patient [15], and recently, this cell line has been widely used as a model system for evaluating

cancer cell apoptosis [17] and [18]. As a result, HAG was found to induce apoptotic body formation stronger than AG (Fig. 3A), and HAG significantly suppressed AGS cell proliferation from a lower concentration, whereas AG did not suppress cell proliferation at any concentration (Fig. 3B). In addition, we prepared water and methanol eluates from HAG (Fig. 3C) to assess their cell proliferation ability, as well as to find out the main active component. As a result, the methanol eluate almost completely suppressed the cell proliferation from a concentration of 50 μg/mL, although there was no suppression in the water eluate (Fig. 3D). It has been shown Acetophenone that a high concentration of the ginsenosides protopanaxidiol and protopanaxatriol learn more are eluted in methanol fraction [19], suggesting that this antiproliferating activity of the methanol eluate was due to ginsenosides. Next, we examined the antiproliferating efficacies of ginsenosides Rb1 and Re with or without heat-processing, because the amounts of these ginsenosides were decreased

markedly after heat-processing in AG than in other ginsenosides (Fig. 2A). Both ginsenosides Re and Rb1 showed no efficacy in cancer cell proliferation (Fig. 4) without heat-processing. However, heat processed-Rb1 significantly suppressed cell proliferation from a lower concentration (Fig. 4A), and this result was similarly observed in the case of methanol elute (Fig. 3D). By contrast, ginsenoside Re showed a weak efficacy even at a high concentration of 100 μg/mL (Fig. 4B). Therefore, the major active component of HAG was considered to be related to heat-processed ginsenoside Rb1. From the HPLC analysis of ginsenoside Rb1, prior to and after heat-processing, ginsenoside Rb1 was transformed into ginsenosides 20(S,R)-Rg3, Rk1, and Rg5 after heat-processing at 120°C ( Fig. 4Cand D) as shown in AG ( Fig. 2). We previously reported that ginsenoside Re gradually transformed into less polar ginsenosides Rg2, Rg6, and F4 upon heat-processing [7].